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αi kinase chimeras  (Addgene inc)


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    Structured Review

    Addgene inc αi kinase chimeras
    Screening <t>αI</t> kinase substitutions. The 118 αI kinase sequences are shown along with a multiple sequence alignment and fitness scores. The fitness scores are shown for the human <t>BTK</t> <t>SH2</t> genetic background, the BMX-H SH2 genetic background, and the difference between these two backgrounds (BTK values subtracted from the BMX-H values). Error bars represent SEM and points represent the individual replicate values. For the difference values, error bars are determined using the SE propagation formula.
    αi Kinase Chimeras, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1i+kinase+chimeras/pHIV-EGFP+(Plasmid+%2321373)/pmc12541323-190-20-26
    Average 94 stars, based on 101 article reviews
    αi kinase chimeras - by Bioz Stars, 2026-08
    94/100 stars

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    1) Product Images from "BTK autoinhibition analyzed by high-throughput swaps of SH2 domains"

    Article Title: BTK autoinhibition analyzed by high-throughput swaps of SH2 domains

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.2502688122

    Screening αI kinase substitutions. The 118 αI kinase sequences are shown along with a multiple sequence alignment and fitness scores. The fitness scores are shown for the human BTK SH2 genetic background, the BMX-H SH2 genetic background, and the difference between these two backgrounds (BTK values subtracted from the BMX-H values). Error bars represent SEM and points represent the individual replicate values. For the difference values, error bars are determined using the SE propagation formula.
    Figure Legend Snippet: Screening αI kinase substitutions. The 118 αI kinase sequences are shown along with a multiple sequence alignment and fitness scores. The fitness scores are shown for the human BTK SH2 genetic background, the BMX-H SH2 genetic background, and the difference between these two backgrounds (BTK values subtracted from the BMX-H values). Error bars represent SEM and points represent the individual replicate values. For the difference values, error bars are determined using the SE propagation formula.

    Techniques Used: Sequencing



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    αi kinase chimeras  (Addgene inc)
    94
    Addgene inc αi kinase chimeras
    Screening <t>αI</t> kinase substitutions. The 118 αI kinase sequences are shown along with a multiple sequence alignment and fitness scores. The fitness scores are shown for the human <t>BTK</t> <t>SH2</t> genetic background, the BMX-H SH2 genetic background, and the difference between these two backgrounds (BTK values subtracted from the BMX-H values). Error bars represent SEM and points represent the individual replicate values. For the difference values, error bars are determined using the SE propagation formula.
    αi Kinase Chimeras, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1i+kinase+chimeras/pHIV-EGFP+(Plasmid+%2321373)/pmc12541323-190-20-26
    Average 94 stars, based on 1 article reviews
    αi kinase chimeras - by Bioz Stars, 2026-08
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Screening αI kinase substitutions. The 118 αI kinase sequences are shown along with a multiple sequence alignment and fitness scores. The fitness scores are shown for the human BTK SH2 genetic background, the BMX-H SH2 genetic background, and the difference between these two backgrounds (BTK values subtracted from the BMX-H values). Error bars represent SEM and points represent the individual replicate values. For the difference values, error bars are determined using the SE propagation formula.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: BTK autoinhibition analyzed by high-throughput swaps of SH2 domains

    doi: 10.1073/pnas.2502688122

    Figure Lengend Snippet: Screening αI kinase substitutions. The 118 αI kinase sequences are shown along with a multiple sequence alignment and fitness scores. The fitness scores are shown for the human BTK SH2 genetic background, the BMX-H SH2 genetic background, and the difference between these two backgrounds (BTK values subtracted from the BMX-H values). Error bars represent SEM and points represent the individual replicate values. For the difference values, error bars are determined using the SE propagation formula.

    Article Snippet: The next day (day 2), cells were transfected with a mixture of transfer plasmid containing the SH2-BTK chimeras or the αI kinase chimeras (modified from pHIV-EGFP, Addgene #21373, 5 μg) and two packaging plasmids (pMDG.2, Addgene #12259, 1.25 μg and psPAX2, Addgene #12260, 3.75 μg) in 500 μL of opti-mem (Thermo Fisher) using Lipofectamine LTX (Thermo Fisher) according to the manufacturer’s instructions.

    Techniques: Sequencing